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Chopra, Chirag
- Isolation and Characterization of Microbial Diversity of Soil of Dhanbad Coal Mines using Molecular Approach
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Authors
Affiliations
1 School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, IN
1 School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 3 (2019), Pagination: 1137-1140Abstract
Soil is the richest ecosystem there is, as far as the diversity and populations of microorganisms is concerned. It is known that there could be over ten billion microbes per gram of soil, making soil the ideal ecosystem for mining different genes, ORFs etc. Microbial Diversity forms the lifeline of an ecosystem, interfering, regulating and participating in the biogeochemical activities therein. The present study was conducted to identify key extremophilic species residing in the coal mines of Dhanbad plateau in Bihar, India. Dhanbad plateau soil presents a unique niche, rich in coal and minerals. Soil from the coal mines was analysed through metagenomic as well as culture-dependent approach to enlist the micro-organisms native to the place. The culturable fraction lead to the identification of Klebsiella spp, Enterobacteriaceae, Serratia marcescens, and Providencia spp.Keywords
Microbial Diversity, Molecular techniques, 16S rRNA genes, Dhanbad Plateau, Metagenomics.
References
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- Accessing Genetic Diversity and Phylogenetic Analysis of Microbial Population of Soil from Hygam Wetland of Kashmir Valley
Abstract Views :170 |
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Authors
Mousumi Sarangi
1,
Chirag Chopra
1,
Yusuf Ado Usman
2,
Daljeet Singh Dhanjal
1,
Reena Singh Chopra
1
Affiliations
1 School of Bioengineering and Biosciences, Lovely Professional University, Phagwara -144411, IN
2 Department of Biochemistry, Faculty of Basic Medical Science, Bayero University Kano, NG
1 School of Bioengineering and Biosciences, Lovely Professional University, Phagwara -144411, IN
2 Department of Biochemistry, Faculty of Basic Medical Science, Bayero University Kano, NG
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2323-2326Abstract
Assessing microbial diversity of a region is important for understanding ecosystem functions and processes. The cold wetlands of the Himalayas are relatively unexplored for the microbial diversity. In the present study, one such wetland of the Kashmir region, Hygam Wetland (34°13′30′′- 34°16′4′′N and 74°30′27′′- 74°32′33′′E), was chosen for determination of the microbial diversity. Metagenomic DNA was extracted and 16S r-RNA gene was amplified followed by sequencing and BLASTn analysis of the sequences. Phylogenetic tree of identified sequences was made by pairwise alignment followed by tree generation using Clustal omega. In the BLASTn analysis, similarity with Bacillus safensis, uncultured Lysinibacillus C1A14-2 and uncultured clone 4015B10 was observed. Further, the microbes cultured from Hygam soil were identified using biochemical tests. These included Shigella species, Salmonella choleraesuis, Citrobacter species and Escherichia coli.Keywords
Hygam, Kashmir Wetland, Metagenomics, Microbial Diversity, Phylogenetic Analysis.References
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